Depo-Nitrolase 250 (ProHydrolase)


ProHydrolase is a bio-sufficient supplement designed to build muscle mass and accelerate muscle recovery when used as an ingredient in protein supplements or meal replacements.

buy on amazon

In stock

SKU: 123363 Category:

Product Description

Depo Nitrolase 250 with ProHydrolase is a bio-sufficient supplement designed to build muscle mass and accelerate muscle recovery when used as an ingredient in protein supplements or meal replacements.
Takes full advantage of the availability of essential amino acids for building muscle and improving muscle recovery
Greatly reduces digestive caused when protein is not broken down into the smallest composition.
Maximizes the performance benefit of protein supplements, assisting the body in absorbing more protein rather than excreting it.
Effective at digesting various forms of protein including whey, soy, egg, casein, hemp and pea.


Numerous in vitro studies plus two separate human clinical studies have shown the superior ability of ProHydrolase to quickly break down protein, increase available amino acids in the blood, decrease C-reactive protein levels and decrease the large peptides associated with stomach discomfort that results from consuming protein.

In vitro Testing: Gel Electrophoresis


Discomfort PeptidesProHydrolase breaks down large peptides, reducing the potential for discomfort that often is a result of consuming protein.

Amino Acids Lost
Undigested whey protein will simply be excreted from the body, resulting in wasted amino acids.


Whey breakdown pancreatin

Plant Proteins



Clinical Results

Check out the ProHydrolaseClinical-AdvStudy (2)

Total Amino Acids
Total Amino Acids
C-Reactive Protein


Total amino acid concentrations increased 55mg in the blood over the 270 minutes after ingestion of whey protein isolate with ProHydrolase when compared to taking whey protein isolate alone. This correlates with published results showing the increase of amino acids when taking whey hydrolysate vs. whey protein unprocessed.
CRP levels start at 750 micrograms/liter, and increase to approximately 900 micrograms/liter after consuming whey. With the addition of ProHydrolase, levels decrease to 500 micrograms/liter, and then gradually increase over the 4 hours to 700 micrograms/liter.



What is ProHydrolase?
ProHydrolase is a proprietary blend of proteolytic enzymes, designed to build muscle mass and accelerate muscle recovery when used as an ingredient in protein supplements or meal replacements. Benefits include: 1) digests protein into bio-usable form to increase protein absorption, 2) maximizes availability of amino acids, 3) greatly reduces stomach discomfort (nausea, bloating, cramping) associated with consuming high amounts of protein, 4) effective at digesting whey, casein, egg, soy, hemp and pea proteins.

How does it work?
ProHydrolase helps break down protein efficiently and completely so that the body can maximize the absorption of amino acids into the blood stream, and reduce the exposure time to whey peptides that can cause discomfort. Consumption of whey protein with ProHydrolase significantly raises the level of amino acids in the blood, compared to whey isolate alone. Among those amino acids are the branch chain amino acids, which have been shown to play a vital role in muscle synthesis and recovery.

Is it non-GMO?
The microorganisms used to produce this enzyme product are not genetically engineered organisms as defined by the National Organic Standards Board. Genetically engineered is defined as follows:
Made with techniques that alter the molecular or cellular biology of an organism by means that are not possible under natural conditions or processes. Genetic engineering includes recombinant DNA, cell fusion, micro-and macro-encapsulation, gene deletion and doubling, introducing a foreign gene, and changing the position of genes. It shall not include breeding, conjugation, fermentation, hybridization, in-vitro fertilization, and tissue culture.

What is the recommended dosage?
Dosage: 10mg of ProHydrolase per gram of protein. It is important to note that most containers of protein supplements are not 100% pure protein, so the calculation must be adjusted to account for the amount of pure protein in the container.

Are there clinical studies that prove it works or reference studies we can review?
Two separate human clinical studies have been performed, and numerous reference studies are available. Contact us for more details on these studies.

I saw one product with 170,000 HUT and yours only has 3500 HUT, does that mean the 170,000 HUT is better?  Absolutely not!  Don’t be fooled by MORE is better trick.  Remember, Depo 250 with Prohydrolase contains 3500 HUT per 10mg.  Plus it is one of the only digestive enzyme products with real clinical research.  Most other digestive protein enzyme supplements do not have this.



Net Weight: 90 capsules

An excerpt from Internet Society for Sports Science: 7 March 1998.

For the most comprehensive source on Depo-Nitrolase 250: Click Here

Listen to the webinar on Depo-Nitrolase 250 Click Here


Depo-Nitrolase 250
Coming out with new products is not easy. Well it is if you follow all the fad products. Like the pre-workout powders. Talk about a fad category going hay wire. Anyway coming out with new products is not the easiest thing in the world. Why? Because if you want to create something new in the sports nutrition business, you have to rely on yourself. If not, then you are relying on someone else to make new ingredients. Did you know that not one sports nutrition company has EVER created their own new ingredient? The big sports nutrition companies that you buy from try to make you think that they create their own ingredients, but they do not. Usually, it is some guy in China. All of the major sports nutrition companies pretend they have their own labs and scientists and do their own testing. But this is all smoke and mirrors. They do nothing of the sorts. They rely on people like me to actually invent new sports nutrition ingredients. Thus, the result is that to date nothing as we know it grows muscle. You cannot take a pill 3 times a day and expect to grow muscle. That’s the truth. You can only take products that support and aid in muscle growth. What are these products? The best is protein. Taking in protein puts your body (sometimes) in an anabolic state. After that dietary fat. Dietary fats again place the body into an anabolic state.

But to reiterate these products do not “grow “muscle. If I fed you 500 grams of protein a day and you just did nothing all day long, you’d just get fat. Thus, when coming out with a new product you want to create a product that directly relates to growing muscle as close as possible. I wanted to possibly find a product that would force the body to use more protein and amino acids. Secondly I wanted a product that had some real science behind it,

The name of the product is called Depo-Nitrolase 250. It is a high potency blend of protease enzymes that allow for complete protein assimilation into amino acids within 90 minutes of consumption. Now this product is not comprised of your ordinary protease enzymes. These enzymes were formulated, researched, and then tested to see how well they break down protein and how well your body absorbs them. Secondly, compared to other enzymes Depo-Nitrolase 250 kicks their butts. See the study comparing it with Aminogen, (the well-known enzyme that most sports nutrition companies use in their proteins if they do want to use an enzyme.) Back to what I was saying, Depo-Nitrolase 250 allows for complete protein assimilation.

Re-read what I just said. Complete protein assimilation. Ok so let me explain where my train of thought is when I created this product.. Everyone is different and everyone has different protein absorption rates normally. If your body is not going to use the protein, the protein is excreted as urine or feces. The body will take the amino acids from the food you eat and use it accordingly. It takes what it needs. If you are natural and not a steroid user, the body will take what it has always taken. It is simply not going to take more aminos to force itself to grow muscle. But when you take the Depo-Nitrolase 250 you’re getting your body to take more than it normally would.

The body does not like muscle mass. The more muscle mass you have on your frame the more energy it takes for you to live. Why do you think it’s so easy to lose muscle as opposed it is to gain? I dare you to stop working out for 30 days! You’ll see yourself shrink and get weaker real quick! Thus we continue to body build because we know that if we stopped we would lose muscle mass. And that is a fact. So we continue to body build and continue to eat a high protein diet. But day after day, the body adjusts. If your body did not adjust you would continue to make lean muscle gains. But you don’t. Everyone has a maximum amount of muscle they can build. If there was not a maximum amount you’d have 500lb muscular bodybuilders. These guys that take huge amounts of steroids and growth hormones have no stopping point in regards to how much juice and GH they are using. The more drugs they take, the bigger they get. But even they have their limits on how big they can get. Again even with all the drugs they take, I haven’t seen a 400lbs juicer walking around, even the most heavily and genetically gifted bodybuilders cannot get beyond a lean muscular 300lbs. But we do not recommend or suggest taking steroids, but we do recommend taking a product that can “trick” the body to use more protein and hopefully grow muscle.

What we are looking to do is naturally force the body to use more protein. And that my friend equates to muscle growth. No if’s and’s or but’s. Make the body use more protein, of course partake in weight training, and you’re going to grow muscle. Therefore like this statement above…….


With Depo-Nitrolase 250, we are enhancing the activation rate of the enzymes systems in our body thus increasing protein synthesis and inhibiting protein degradation.

You want to use Depo-Nitrolase 250 with every protein meal.



Lab Guarantee